Journal: Poultry Science

Article Title: Targeting TatD nuclease and the MAPK Pathway: Luteolin multifaceted approach against Mycoplasma gallisepticum infection

doi: 10.1016/j.psj.2026.106426

Figure Lengend Snippet: TAT-TatD recombinant protein causes pyroptosis of cells. (A-G) The effect of TAT-TatD recombinant protein (2.5 μg/mL) on inflammatory factors within HD-11 cells ( n = 3), (A-C) The gene expression of TNF-α, IL-6, IL-1β, (D-G) The protein expression of TNF-α, IL-6, IL-1β (with GAPDH serving as the internal control gene). (H—N) The effect of TAT-TatD recombinant protein (2.5 μg/mL) on pyroptosis factors within HD-11 cells ( n = 3), (H-J) The gene expression of Caspase-1, GSDMA, IL-18, (K-N) The protein expression of Caspase-1, GSDMA, and IL-18 (with GAPDH serving as the internal control gene). (O-T) The effect of TAT-TatD recombinant protein (2.5 μg/mL) on cGAS-STING signaling pathway within HD-11 cells ( n = 3). (O-P) The gene expression of cGAS and STING, (Q-T) The protein expression of cGAS, STING, and p-STING (with GAPDH serving as the internal control gene). (U) Immunofluorescence image of the cGAS-STING pathway, (V) Analysis of the fluorescence intensity of cGAS, (W) Analysis of the fluorescence intensity of p-STING.

Article Snippet: TNF-α , Bioss (bsm-33207 M) , 1:1000.

Techniques: Recombinant, Gene Expression, Expressing, Control, Immunofluorescence, Fluorescence

Journal: Poultry Science

Article Title: Targeting TatD nuclease and the MAPK Pathway: Luteolin multifaceted approach against Mycoplasma gallisepticum infection

doi: 10.1016/j.psj.2026.106426

Figure Lengend Snippet: LUT ameliorates pyroptosis induced by TAT-TatD recombinant protein in HD-11 cells via the cGAS-STING pathway. (A-G) The effect of LUT (16, 32, 64 μM) on inflammatory factors after treatment with TAT-TatD recombinant protein (2.5 μg/mL) ( n = 3), (A-C) The gene expression of TNF-α, IL-6, IL-1β, (D-G) The protein expression of TNF-α, IL-6, IL-1β (with GAPDH serving as the internal control gene). (H—N) The effect of LUT (16, 32, 64 μM) on pyroptosis factors after treatment with TAT-TatD recombinant protein (2.5 μg/mL) ( n = 3), (H-J) The gene expression of Caspase-1, GSDMA, IL-18, (K-N) The protein expression of Caspase-1, GSDMA, and IL-18 (with GAPDH serving as the internal control gene). (O-T) The effect of LUT (16, 32, 64 μM) on cGAS-STING signaling pathway after treatment with TAT-TatD recombinant protein (2.5 μg/mL) ( n = 3). (O-P) The gene expression of cGAS and STING, (Q-T) The protein expression of cGAS, STING, and p-STING (with GAPDH serving as the internal control gene).

Article Snippet: TNF-α , Bioss (bsm-33207 M) , 1:1000.

Techniques: Recombinant, Gene Expression, Expressing, Control

Journal: Bioactive Materials

Article Title: Mossy-textured hydroxyapatite-modified poly (lactic-co-glycolic acid) microspheres promote collagen regeneration via calcium/TGF-β and chemokine signaling pathways in soft tissue augmentation

doi: 10.1016/j.bioactmat.2025.12.028

Figure Lengend Snippet: The regulatory effects of CaHA/PLGA microspheres on macrophages and ADSCs in vitro. (A, B) CLSM images and RFI of CD86 and CD206 expression in RAW264.7 cells co-cultured with microspheres for 2 days (n = 3). (C–F) Relative mRNA expression levels of inflammation-related genes TNF-α, IL-6, TGF-β1, and FGF-2 in RAW264.7 cells (n = 3). (G, H) Sirius red staining images and quantitative analysis (n = 3) of collagen deposition of ADSCs co-cultured with CaHA/PLGA microspheres for 3 and 7 days. (I–K) Relative mRNA expression levels of TGF-β1, FGF-2, and PDGF-A in ADSCs after 3 and 7 days of co-culture with CaHA/PLGA microspheres (n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.

Article Snippet: CD86 polyclonal antibody (1:200, 13395-1-AP, Proteintech), CD206 (1:200, 18704-1-AP, Proteintech), and TNF-α recombinant antibody (1:200, 80258-6-RR, Proteintech) were used as primary antibodies, incubated overnight at 4 °C.

Techniques: In Vitro, Expressing, Cell Culture, Staining, Co-Culture Assay

Journal: Bioactive Materials

Article Title: Mossy-textured hydroxyapatite-modified poly (lactic-co-glycolic acid) microspheres promote collagen regeneration via calcium/TGF-β and chemokine signaling pathways in soft tissue augmentation

doi: 10.1016/j.bioactmat.2025.12.028

Figure Lengend Snippet: Evaluation of soft tissue filling and inflammatory response in rats. (A, B) Schematic diagram of the soft tissue filling experiment and injection sites, with at least 2 cm spacing between sites. (C) Photographs of subcutaneous soft tissue filling at 2, 4, 8, and 12 weeks. The white circles indicate the soft tissue filling areas. (D) H&E staining of the filled sites. (E, F) Immunofluorescence images and RFI of TNF-α and TGF-β expression at 2 weeks post-filling (n = 6). (G, H) Immunofluorescence images and RFI of CD86 and CD206 expression at 2 weeks post-filling (n = 6). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.

Article Snippet: CD86 polyclonal antibody (1:200, 13395-1-AP, Proteintech), CD206 (1:200, 18704-1-AP, Proteintech), and TNF-α recombinant antibody (1:200, 80258-6-RR, Proteintech) were used as primary antibodies, incubated overnight at 4 °C.

Techniques: Injection, Staining, Immunofluorescence, Expressing

Journal: bioRxiv

Article Title: Inexpensive High-Throughput Multiplexed Cytokine Detection for Tuberculosis Diagnostics Using Amplified Enzymatic Metallization

doi: 10.64898/2026.01.27.700981

Figure Lengend Snippet: (a) Schematic of immunoassay for cytokine detection. (b) Initial LOD optimization for the IFN-γ assay. (c-e) IFN-γ (n=2), IL-2 (n=5), TNF-α (n=2) dilution curves using recombinant cytokines (f-h) Correlation plots of ELISA absorbance values at 450 nm versus silver darkness values for IFN-γ (f), TNF-α (g), and IL-2 (h). All fit lines are four-parameter logistic sigmoidal curves.

Article Snippet: Recombinant human IFN-γ protein (11725-HNAS), biotinylated anti-human IFN-γ antibody (11725-R209-B), anti-human IFN-γ antibody (11725-R238), HRP-conjugated anti-human IFN-γ antibody (11725-R209-H), recombinant human TNF-α protein (10602-HNAE), biotinylated anti-human TNF-α antibody (10602-MM08-B), and anti-human TNF-α antibody (10602-MM01) were obtained from SinoBiological.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Inexpensive High-Throughput Multiplexed Cytokine Detection for Tuberculosis Diagnostics Using Amplified Enzymatic Metallization

doi: 10.64898/2026.01.27.700981

Figure Lengend Snippet: (a) Schematic demonstrating the stimulation groups and controls. Dilution curves for IFN-γ (b-c), IL-2 (d-e), and TNF-α (f-g) in pooled LTB+ and LTB-clinical supernatant samples. Clinical samples consisted of NIL (negative control), Ag1, Ag2, and Mitogen (positive control) groups (n=2).

Article Snippet: Recombinant human IFN-γ protein (11725-HNAS), biotinylated anti-human IFN-γ antibody (11725-R209-B), anti-human IFN-γ antibody (11725-R238), HRP-conjugated anti-human IFN-γ antibody (11725-R209-H), recombinant human TNF-α protein (10602-HNAE), biotinylated anti-human TNF-α antibody (10602-MM08-B), and anti-human TNF-α antibody (10602-MM01) were obtained from SinoBiological.

Techniques: Negative Control, Positive Control

Journal: bioRxiv

Article Title: Inexpensive High-Throughput Multiplexed Cytokine Detection for Tuberculosis Diagnostics Using Amplified Enzymatic Metallization

doi: 10.64898/2026.01.27.700981

Figure Lengend Snippet: Individual participant silver darkness values for IFN-γ (a), IL-2 (b), and TNF-α (c) detection from NIL, Ag1, Ag2, and Mitogen samples of IGRA+ and IGRA-patients using a sample dilution of 1:10. Average of n=2 repeats was plotted for each sample.

Article Snippet: Recombinant human IFN-γ protein (11725-HNAS), biotinylated anti-human IFN-γ antibody (11725-R209-B), anti-human IFN-γ antibody (11725-R238), HRP-conjugated anti-human IFN-γ antibody (11725-R209-H), recombinant human TNF-α protein (10602-HNAE), biotinylated anti-human TNF-α antibody (10602-MM08-B), and anti-human TNF-α antibody (10602-MM01) were obtained from SinoBiological.

Techniques:

Journal: bioRxiv

Article Title: Inexpensive High-Throughput Multiplexed Cytokine Detection for Tuberculosis Diagnostics Using Amplified Enzymatic Metallization

doi: 10.64898/2026.01.27.700981

Figure Lengend Snippet: (a) Schematic of laser-cut PDMS microwell array reversibly assembled on a polystyrene petri dish and workflow of enzymatically amplified silver metallization using Biotin-BSA and poly-HRP-SA. (b) Cell-phone image of silver metallized wells after assay. (c) Biotin-BSA dilution curve obtained from cell-phone image silver darkness quantification in ImageJ. (d) Assay stack for initial biotinyl tyramide signal amplification testing using biotinylated anti-IFN-γ as capture. (e) Dilution curve of silver darkness of increasing biotinylated anti-IFN-γ as target and 10 µg/mL, 1 µg/mL, and 0 µg/mL biotinyl tyramide. All assays were repeated three (n=3) times and fit lines are four-parameter logistic sigmoidal curves.

Article Snippet: Recombinant human IFN-γ protein (11725-HNAS), biotinylated anti-human IFN-γ antibody (11725-R209-B), anti-human IFN-γ antibody (11725-R238), HRP-conjugated anti-human IFN-γ antibody (11725-R209-H), recombinant human TNF-α protein (10602-HNAE), biotinylated anti-human TNF-α antibody (10602-MM08-B), and anti-human TNF-α antibody (10602-MM01) were obtained from SinoBiological.

Techniques: Amplification

Journal: bioRxiv

Article Title: Inexpensive High-Throughput Multiplexed Cytokine Detection for Tuberculosis Diagnostics Using Amplified Enzymatic Metallization

doi: 10.64898/2026.01.27.700981

Figure Lengend Snippet: (a) Schematic of immunoassay for cytokine detection. (b) Initial LOD optimization for the IFN-γ assay. (c-e) IFN-γ (n=2), IL-2 (n=5), TNF-α (n=2) dilution curves using recombinant cytokines (f-h) Correlation plots of ELISA absorbance values at 450 nm versus silver darkness values for IFN-γ (f), TNF-α (g), and IL-2 (h). All fit lines are four-parameter logistic sigmoidal curves.

Article Snippet: Recombinant human IFN-γ protein (11725-HNAS), biotinylated anti-human IFN-γ antibody (11725-R209-B), anti-human IFN-γ antibody (11725-R238), HRP-conjugated anti-human IFN-γ antibody (11725-R209-H), recombinant human TNF-α protein (10602-HNAE), biotinylated anti-human TNF-α antibody (10602-MM08-B), and anti-human TNF-α antibody (10602-MM01) were obtained from SinoBiological.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Inexpensive High-Throughput Multiplexed Cytokine Detection for Tuberculosis Diagnostics Using Amplified Enzymatic Metallization

doi: 10.64898/2026.01.27.700981

Figure Lengend Snippet: (a) Schematic demonstrating the stimulation groups and controls. Dilution curves for IFN-γ (b-c), IL-2 (d-e), and TNF-α (f-g) in pooled LTB+ and LTB-clinical supernatant samples. Clinical samples consisted of NIL (negative control), Ag1, Ag2, and Mitogen (positive control) groups (n=2).

Article Snippet: Recombinant human IFN-γ protein (11725-HNAS), biotinylated anti-human IFN-γ antibody (11725-R209-B), anti-human IFN-γ antibody (11725-R238), HRP-conjugated anti-human IFN-γ antibody (11725-R209-H), recombinant human TNF-α protein (10602-HNAE), biotinylated anti-human TNF-α antibody (10602-MM08-B), and anti-human TNF-α antibody (10602-MM01) were obtained from SinoBiological.

Techniques: Negative Control, Positive Control

Journal: bioRxiv

Article Title: Inexpensive High-Throughput Multiplexed Cytokine Detection for Tuberculosis Diagnostics Using Amplified Enzymatic Metallization

doi: 10.64898/2026.01.27.700981

Figure Lengend Snippet: Individual participant silver darkness values for IFN-γ (a), IL-2 (b), and TNF-α (c) detection from NIL, Ag1, Ag2, and Mitogen samples of IGRA+ and IGRA-patients using a sample dilution of 1:10. Average of n=2 repeats was plotted for each sample.

Article Snippet: Recombinant human IFN-γ protein (11725-HNAS), biotinylated anti-human IFN-γ antibody (11725-R209-B), anti-human IFN-γ antibody (11725-R238), HRP-conjugated anti-human IFN-γ antibody (11725-R209-H), recombinant human TNF-α protein (10602-HNAE), biotinylated anti-human TNF-α antibody (10602-MM08-B), and anti-human TNF-α antibody (10602-MM01) were obtained from SinoBiological.

Techniques: